primary human dermal microvascular ecs  (PromoCell)

Journal: Scientific Reports

Article Title: Deciphering influence of donor age on adipose-derived stem cells: in vitro paracrine function and angiogenic potential

doi: 10.1038/s41598-024-73875-x

Figure Lengend Snippet: Cell population definition according to surface marker.

Article Snippet: Human Dermal Endothelial Cells (EC) from juvenile donor (3yo) were purchased from Promocell and cultivated in EGM-2 (PromoCell, Heidelberg, Germany).

Techniques: Marker

Journal: Scientific Reports

Article Title: Deciphering influence of donor age on adipose-derived stem cells: in vitro paracrine function and angiogenic potential

doi: 10.1038/s41598-024-73875-x

Figure Lengend Snippet: Quantification of cell populations in the SVF and their repartition among CD45- and CD45 + populations. n = 6 donors were used in each age category. * p value < 0,05 indicated in bold . Unpaired Wilcoxon test was used to test the significance of ASC and pericytes populations. Unpaired student t-test was used to test the significance of other cell populations.

Article Snippet: Human Dermal Endothelial Cells (EC) from juvenile donor (3yo) were purchased from Promocell and cultivated in EGM-2 (PromoCell, Heidelberg, Germany).

Techniques:

Journal: Scientific Reports

Article Title: Deciphering influence of donor age on adipose-derived stem cells: in vitro paracrine function and angiogenic potential

doi: 10.1038/s41598-024-73875-x

Figure Lengend Snippet: ( A ) Diversity of sprouting of endothelial cells from dextran beads stained with phalloidin and Hoechst to visualize actin and nucleus, respectively (original magnification 10x, bar, 200 μm). ( B ) Total sprouts length with co-culture of EC with ASC (EC/ASC; dark grey) and fibroblasts (EC/Fb; light grey) as stimulating cells. ( B ) Total sprouts length with co-culture of EC with either ASC (EC/ASC; dark grey) and fibroblasts (EC/Fb; light grey) as stimulating cells. ( C ) Total sprouts length according to co-culture of EC with ASC from young (EC/ASC y; blue) or old (EC/ASC o; red) donors as stimulating cells. n = 5 donors for ASC young, n = 6 donors for ASC old, n = 1 donor for fibroblasts, experiments were conducted in 4 replicates. Unpaired Wilcoxon test was used to test the significance. * P value < 0.05.

Article Snippet: Human Dermal Endothelial Cells (EC) from juvenile donor (3yo) were purchased from Promocell and cultivated in EGM-2 (PromoCell, Heidelberg, Germany).

Techniques: Staining, Co-Culture Assay

Journal: bioRxiv

Article Title: The unconventional Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane

doi: 10.1101/2023.08.11.552954

Figure Lengend Snippet: (A) IF localisation of endogenous Myo1c (green), actin (magenta) and VWF (blue) in unstimulated or PMA (100 ng/ml) stimulated HUVEC in the presence and absence of 1 µM of the actin poison cytochalasin E (CCE). Scale bar 10 µm. Inset 1 µm. Arrows indicate polarised localisation of Myo1c. Myo1c is recruited independently of actin but was dependent on stimulation with PMA. (B) Co-expression of GFP-Myo1c (green) and LifeAct-Ruby (magenta) demonstrated col-localisation with actin at the leading edge. (C) GFP-Myo1c encapsulates WPB post-fusion as determined by live cell confocal imaging of HUVEC co-expressing a GFP-Myo1c and the WPB fusion marker P.sel.lum.mCherry. HUVEC were stimulated with 100 ng/ml PMA and imaged live with a confocal laser scanning microscope. Z-stacks at a spacing of 0.5 µm were acquired continuously. Scale bar 1 µm. Arrow indicates point of collapse/fusion of vesicle (D) Recruitment kinetics of GFP-Myo1c (Mean intensity fluorescence). Dashed grey line = fusion/vesicle collapse. (E) Live cell imaging of LifeAct-GFP and P.sel.lum.mCherry expressing microvascular endothelial cells indicated the utility of actin rings to expel VWF following stimulation. Scale bar 10 µm. Inset 1 µm. (F) IF analyses of endogenous Myo1c in HDMEC that were left untreated or stimulated with PMA, CCE or CCE and PMA. White arrows illustrate where GFP-Myo1c is recruited to WPB. Scale bar 10 µm

Article Snippet: Human Umbilical Vein ECs (HUVEC) (Cat: 12203) and Human Dermal Microvascular ECs (HDMEC) (Cat: 12212) were purchased from PromoCell.

Techniques: Expressing, Imaging, Marker, Laser-Scanning Microscopy, Fluorescence, Live Cell Imaging